Official Opentrons Protocol API for OT-2 and Flex robots. Use when writing protocols specifically for Opentrons hardware with full access to Protocol API v2 features. Best for production Opentrons protocols, official API compatibility. For multi-vendor automation or broader equipment control use pylabrobot.
# Opentrons Integration ## Overview Opentrons is a Python-based lab automation platform for Flex and OT-2 robots. Write Protocol API v2 protocols for liquid handling, control hardware modules (heater-shaker, thermocycler), manage labware, for automated pipetting workflows. ## When to Use This Skill This skill should be used when: - Writing Opentrons Protocol API v2 protocols in Python - Automating liquid handling workflows on Flex or OT-2 robots - Controlling hardware modules (temperature, magnetic, heater-shaker, thermocycler) - Setting up labware configurations and deck layouts - Implementing complex pipetting operations (serial dilutions, plate replication, PCR setup) - Managing tip usage and optimizing protocol efficiency - Working with multi-channel pipettes for 96-well plate operations - Simulating and testing protocols before robot execution ## Core Capabilities ### 1. Protocol Structure and Metadata Every Opentrons protocol follows a standard structure: ```python
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